Figure 4: Surface markers expression. Dendritic cells pulsed with different TCL preparations were harvested, stained for CD83 and CD86, and analyzed by flow cytometry. (A) Representative flow cytometry profile (one of three experiments); (B) the percentage of stained cells with CD83 and CD86 for different TCL preparation strategies. Data are expressed as mean ± SD, n = 3. TCL, Tumor cell lysates.