Figure 5: The effect of oxypeucedanin and isoimperatorin on (A) Bax, (B) Bcl-2 mRNA expression, and (C) Bax/Bcl-2 in PC12 cells. Cells were pretreated with oxypeucedanin and isoimperatorin (80 μg/mL) for 2 h and 21 h, respectively, before exposure to DOX at 5 μM of. Normalization relative to B-actin was performed. Levels of mRNA are expressed relative to the control cells as the mean ± SEM values derived from three independent experiments. *P < 0.05 and ***P < 0.001 indicate significant differences compared to the control, ##P < 0.01 and ###P < 0.001 versus DOX-treated group. DOX, Doxorubicin; ISO, isoimperatorin; OXY, oxypeucedanin.