Figure 4: Effect of oxypeucedanin and isoimperatorin on DOX-induced mitochondrial membrane potential collapse. Cells were pretreated with oxypeucedanin and isoimperatorin at the same concentration of 80 μg/mL for 2 h and 21 h, respectively, before exposure to DOX at 5 μM. The graph is showing the changes in MMP as represented by the mean fluorescence intensity of rhodamine 123. ***P < 0.001 Indicates significant difference compared to the control group and ###P < 0.001 versus DOX-treated group. DOX, Doxorubicin; ISO, isoimperatorin; OXY, oxypeucedanin.