The design and evaluation of the antimicrobial activity of a novel conjugated penta-ultrashort antimicrobial peptide in combination with conventional antibiotics against sensitive and resistant strains of S. aureus and E. coli.

Rula Darwish; Ammar Almaaytah; Ali Salama

doi:10.4103/1735-5362.359429

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ORIGINAL ARTICLE

Year : 2022 | Volume : 17 | Issue : 6 | Page : 612-620

The design and evaluation of the antimicrobial activity of a novel conjugated penta-ultrashort antimicrobial peptide in combination with conventional antibiotics against sensitive and resistant strains of S. aureus and E. coli.

Rula Darwish¹, Ammar Almaaytah², Ali Salama³
¹ Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, 11942, Amman, Jordan., Jordan
² Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan., Jordan
³ Faculty of Pharmacy, Middle East University, Amman, Jordan., Jordan

Correspondence Address:
Ammar Almaaytah
Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Jordan
Rula Darwish
Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, 11942, Amman, Jordan.
Jordan

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/1735-5362.359429

Background and purpose: Antimicrobial resistance still constitutes a major health concern to the global human population. The development of new classes of antimicrobial agents is urgently needed to thwart the continuous emergence of highly resistant microbial pathogens. Experimental approach: In this study, we have rationally designed a novel conjugated ultrashort antimicrobial peptide. The peptide named naprolyginine was challenged against representative strains of wild-type and multidrug-resistant bacteria individually or in combination with individual antibiotics by employing standard antimicrobial and checkerboard assays. Findings / Results: Our results displayed that the peptide exhibits potent synergistic antimicrobial activity against resistant strains of gram-positive and gram-negative bacteria when combined with individual antibiotics. Additionally, the peptide was evaluated for its hemolytic activity against human red blood cells and displayed negligible toxicity. Conclusion and implications: Naprolyginine could prove to be a promising candidate for antimicrobial drug development.

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Antimicrobial peptides; Bacteria; Drug design; Hemolysis; Naproxen

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